The activities of the serum and liver γ-glutamyl trawnspeptidase(γ-GTP) were studied in cholestasis induced by common bile duct(CBD) ligation and chronic ethanol intoxication and in cholestasis before acute ethanol intoxication to establish the
biochemical background of alcoholic hazard in hepatobiliary disease.
There was a significant increase in the hepatic microsomal γ-GTP activity of rats treated with only chronic ethanol intoxication. In the group with CBD ligation after chronic ethanol intoxication, the activities of serum and hepatic microsomal
γ-GTP
increased more markedly than in the group with only CBD ligation. However, the activity of the hepatic mitochondrial γ-GTP showed a significant decrease than in the group with only CBD ligation.
On the other hand, in the group with CBD ligation before acute ethanol intoxication, the activity of hepatic mitochondrial γ-GTP decreased more markedly than in the group with only CBD ligation.
According to the above results, the liver microsomalγ-GTP seems to be an enzyme which increase in its activity in chronic ethanol intoxication with cholestasis more than in cholestasis And the liver mitochondrial γ-GTP seems to be an enzyme
which
decrease in its activities in both acute and chronic ethanol intoxication with cholestasis more than in cholestasis. The causes of the increase or the decrease seem to be the development or the retardment of biosynthesis. Especially, when the
chronic
ethanol intoxication with cholestasis occurred, the activity of serum γ-GTP was higher than that of the cholestasis because of increased liver cell damage, which causes the enzyme to leak into the blood in great quantity.
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